| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Emma Albrecht | 18 | MIMLA | 55.12 | AAAA | 798 |
| 2 | Hannah Gurgel | 17 | MIPCC | 56.78 | AAA | 730 |
| 3 | Kailey Bachmann | 16 | MIBBA | 56.88 | AAA | 740 |
| 4 | Natalie Mazur | 15 | MIWAVE | 56.97 | AAA | 753 |
| 5 | Miranda Phelps | — | D1BELL | 57.03 | — | — |
| 6 | Yahanna Silva Castro | — | D1AAP | 57.29 | AAA | 741 |
| 7 | Harper Feyen | — | D1AAP | 57.52 | AAA | 702 |
| 8 | Maria Baldwin | — | D1SAL | 57.63 | — | — |
| 9 | Hannah King Landmark | — | D1SAL | 58.09 | — | — |
| 10 | Dalila Brown | — | D1JEN | 58.71 | — | — |
| 11 | Polina Park | — | D1GRFH | 58.92 | — | — |
| 12 | Yufan Wu | — | D1NOVI | 59.64 | — | — |
| 13 | Claire Patrick | 15 | MISWYS | 59.76 | AA | 653 |
| — | Piper Smith | — | D1WBLM | 59.78 | — | — |
| 14 | Mallory Shaver | — | D1GB | 59.93 | — | — |
| 15 | Avni Rai | — | D1NOVI | 59.95 | — | — |
| 16 | Annabelle Thompson | — | D1HMIL | 1:00.10 | — | — |
| — | Yan Adler | — | D1AAP | 1:00.13 | AA | 626 |
| — | Bryn Cusack | — | D1WBLM | 1:00.28 | — | — |
| — | Delanie Rimes | — | D1KZC | 1:00.34 | — | — |
| — | Olivia Buford | 16 | MIUSSC | 1:00.36 | AA | 619 |
| — | Evie Melancon | — | D1LSTE | 1:00.88 | — | — |
| — | Paige Davis | — | D1ZEE | 1:00.91 | — | — |
| — | Abigail Sutton | — | D1WLC | 1:01.43 | — | — |
| — | Juri Aala | — | D1LKOR | 1:01.80 | — | — |
| — | Beya Weslati | 15 | MILIFE | 1:03.50 | A | 544 |