| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Abigail Corbett | 12 | FLSRA | 40.94 | B | 410 |
| 2 | Matthew Wilkinson | 12 | FLWOJ | 41.11 | — | 364 |
| 3 | Addison Evans | 12 | FLEAJ | 41.56 | B | 392 |
| 4 | Lily Hansen | 11 | FLSRA | 41.84 | — | 436 |
| 5 | Samantha Perry | 11 | FLBSS | 44.14 | — | 371 |
| 6 | Emilia Dupee | 12 | FLEAJ | 45.80 | — | 293 |
| 7 | Addison Jenkins | 10 | FLEAJ | 46.02 | — | 383 |
| 8 | Noah Ditmer | 11 | GASFY | 46.33 | — | 320 |
| 9 | Sarah Wilkinson | 8 | FLEAJ | 47.09 | — | — |
| 10 | Sophia Gumbayan | 10 | FLSRA | 52.31 | — | 261 |
| 11 | Kellan Burge | 9 | FLEAJ | 52.96 | — | 289 |
| 12 | Anthony Pletenets | 11 | FLEAJ | 55.67 | — | 184 |
| 13 | Elijah Showalter | 11 | FLEAJ | 1:00.82 | — | 141 |
| 14 | Celia Haire | 8 | FLEAJ | 1:03.11 | — | — |