| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Eli Stoll | 18 | OHGCST | 56.00 | — | — |
| 2 | Zachary Stump | 18 | MDNAVY | 56.81 | — | — |
| 3 | Grant Kennedy | 17 | OHUN | 1:00.49 | — | — |
| 4 | John Wion | 16 | OHWAC | 1:02.78 | — | — |
| 5 | John Boyle | 14 | OHUPA | 1:03.89 | BB | — |
| 6 | Reid Caswell | 16 | CATROJ | 1:04.79 | — | — |
| 7 | Owen Thomas | 15 | OHWAC | 1:05.15 | — | — |
| 8 | Andy Li | 15 | OHGCST | 1:05.66 | — | — |
| 9 | Mark Petrowsky | 17 | OHDOB | 1:05.69 | — | — |
| 10 | Clark Pabst | 16 | OHUASC | 1:05.92 | — | — |
| 11 | Ryan Nichols | 16 | OHUN | 1:06.08 | — | — |
| 12 | Adam Wiest | 15 | OHWAC | 1:06.25 | — | — |
| 13 | Ava Snider | 17 | OHUASC | 1:06.45 | — | — |
| 14 | Staci Nation | 18 | OHWAC | 1:09.09 | — | — |
| 15 | Jin-jae Robinson | 15 | OHWAC | 1:09.60 | — | — |
| 16 | Allison Funge | 14 | OHUASC | 1:10.17 | A | — |
| 17 | Audrey Butcher | 17 | OHWAC | 1:10.34 | — | — |
| 18 | Samuel Luxhoj | 15 | OHUPA | 1:10.36 | — | — |
| 19 | Nora Nawrocki | 15 | OHUPA | 1:12.75 | — | — |
| 20 | Ethan Hedges | 13 | OHGCST | 1:12.98 | B | — |
| 21 | Quinton Dardinger | 14 | OHWAC | 1:13.15 | — | — |
| 22 | Abigail Oliphant | 14 | OHUPA | 1:13.43 | BB | — |
| 23 | Ryan Cho | 14 | OHUASC | 1:14.15 | — | — |
| 24 | Araceli Gaver | 16 | OHGCST | 1:15.94 | — | — |
| 25 | Mariella Wilson | 16 | OHWAC | 1:16.56 | — | — |
| 26 | Chayse Neese | 17 | OHGCST | 1:17.37 | — | — |
| 27 | Abigail Marquardt | 17 | OHGCST | 1:24.13 | — | — |
| 28 | Aimee Connell | 14 | OHWAC | 1:31.15 | — | — |