| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Samantha Saggio | — | NCNSS | 41.04 | — | 347 |
| 2 | Hannah Chapman | 10 | NCRCST | 41.63 | — | 332 |
| 3 | Blakeley Hogan | 10 | NCNSS | 42.41 | — | 314 |
| 4 | Julia Studnek | — | NCMSA | 45.62 | — | 252 |
| 5 | Grace Gatehouse | — | NCNSS | 46.18 | — | 243 |
| 1 | Ii, Buttrick | — | NCSAC | 46.30 | — | 290 |
| 6 | Meris Cable | — | NCGG | 49.16 | — | 255 |
| 2 | Noah Legnosky | 9 | NCGG | 51.66 | — | 209 |
| 7 | Reagan Phillips | — | NCGG | 52.57 | — | 165 |
| 3 | Henry Bartscher | — | NCSAC | 52.86 | — | 160 |
| 4 | Wyatt Huss | — | NCSAC | 1:00.86 | — | 128 |
| 5 | William Francis | — | NCSAC | 1:03.93 | — | 90 |
| 6 | James Bartscher | — | NCSAC | 1:05.04 | — | 105 |
| 7 | Gram Morrison | — | NCSAC | 1:05.73 | — | 101 |
| 8 | Mylan Green | 9 | NCSAC | 1:11.18 | — | 80 |
| 9 | Karter Smith | — | NCSAC | 1:16.82 | — | 52 |
| 8 | Lilly Fowler | — | NCRCST | 1:19.04 | — | 61 |