| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Scarlett Lewis | 9 | STLSAC | 43.15 | — | 378 |
| 2 | Isabella Schafer | 10 | STLSAC | 43.33 | — | 295 |
| 3 | Annabelle Dinter | 9 | STLSAC | 43.66 | — | 365 |
| 4 | Nate Thomas | 10 | STLSAC | 44.77 | — | 263 |
| 5 | Makayla Newlin | 9 | STLSAC | 44.94 | — | 334 |
| 6 | Alice Souza | 9 | STLSAC | 47.46 | — | 284 |
| 7 | Lincoln Huennekens | 8 | STGOLD | 47.80 | B | — |
| 8 | Ryan Stacer | 10 | STLSAC | 48.04 | — | 213 |
| 8 | Hailey Flynn | 10 | STLSAC | 48.04 | — | 216 |
| 10 | Claire Tiemann | 10 | STLSAC | 48.06 | — | 216 |
| 11 | Aedan Allen | 8 | STWLOO | 48.71 | — | — |
| 12 | Aubrey Slaughter | 10 | STLSAC | 49.13 | — | 202 |
| 13 | Olivia Maiberger | 10 | STSGA | 49.76 | — | 194 |
| 14 | Cara Nelms | 10 | STSGA | 51.83 | — | 172 |
| 15 | Emery Johnson | 9 | STSGA | 51.91 | — | 217 |
| 16 | Thiago Fragale | 8 | STLSAC | 52.19 | — | — |
| 17 | Samson Schulz | 9 | STSGA | 52.85 | — | 195 |
| 18 | Hunter King | 10 | STLSAC | 54.14 | — | 149 |
| 19 | June Ollestad | 8 | STLSAC | 55.48 | — | — |
| 20 | Levi Bruck | 10 | STLSAC | 55.90 | — | 135 |
| 21 | Aashvi Rathi | 10 | STLSAC | 57.07 | — | 129 |
| 22 | Landon King | 7 | STLSAC | 57.22 | — | — |
| 23 | Darren Xue | 8 | STLSAC | 58.37 | — | — |
| 24 | Vida Reuscher | 7 | STLSAC | 58.50 | — | — |
| 25 | Kian Dharampuria | 9 | STLSAC | 1:01.88 | — | 122 |
| 26 | Abigail Greer | 9 | STSGA | 1:01.93 | — | 128 |
| 27 | Caleb Lockhart | 8 | STLSAC | 1:02.24 | — | — |
| 28 | Hannah Slaughter | 7 | STLSAC | 1:06.47 | — | — |
| 29 | Emily StJacques | 9 | STSGA | 1:07.14 | — | 100 |
| 30 | Kaden Oldham | 10 | STSGA | 1:16.63 | — | 52 |
| 31 | Benjamin Bolkey | 6 | STLSAC | 1:25.92 | — | — |