| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Ella Pearl | 18 | OZUN | 55.77 | — | 845 |
| 2 | Sophie Henriksen | 17 | OZUN | 55.94 | — | 838 |
| 3 | Isabelle Ackley | 14 | FLGCST | 57.08 | — | 825 |
| 4 | Marin Lashley | 16 | OZRSCA | 57.43 | — | 774 |
| 5 | Paige Howell | 18 | OZCSP | 57.58 | — | 768 |
| 6 | Logan Knolhoff | 17 | USUN | 58.20 | — | 744 |
| 7 | Olivia Henderson | 18 | MVCSC | 58.72 | — | 724 |
| 8 | Lydia Grider | 15 | MVUN | 59.67 | — | 699 |
| 10 | Megan Bilsland | 15 | OZUN | 1:00.67 | — | 665 |
| 11 | Henley Schmalz-Turner | 17 | MVUN | 1:00.87 | — | 650 |
| 13 | Ashlyn Moore | 16 | ARUSPA | 1:01.44 | — | 632 |
| 14 | Erin Davis | 15 | MVEKC | 1:02.20 | — | 617 |
| 15 | Madison Colombo | 17 | OZUN | 1:02.22 | — | 609 |
| 17 | Madilyn McNearney | 16 | MVTST | 1:02.59 | — | 598 |
| 18 | Ellie Kipper | 14 | OZUN | 1:02.70 | — | 623 |
| 20 | Ruby Livingston | 16 | MVUN | 1:03.48 | — | 573 |
| 21 | Carly Shaffer | 15 | OZUN | 1:03.56 | — | 578 |
| 22 | Elaina Ginest | 17 | USUN | 1:03.78 | — | 565 |