| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Cory Pham | — | OHXEN | 52.83 | — | — |
| 1 | Brock Gessner | 17 | OHMCY | 53.07 | AA | 656 |
| 2 | Daxton Novitske | 17 | OHCFY | 53.49 | AA | 641 |
| 3 | Liam McClure | 17 | OHDR | 55.47 | A | 575 |
| 4 | Malcolm Hibbert | 17 | OHCFY | 56.53 | A | 543 |
| 2 | Kairav Pai | 16 | OHDTMV | 57.89 | BB | 525 |
| 5 | Ian Massengill | 14 | OHDR | 58.58 | A | 590 |
| 3 | Ian Dean | — | OHDCA | 1:00.30 | — | — |
| 1 | Ciara Dwyer | 18 | OHDR | 1:00.74 | A | 596 |
| 4 | Henry Kaufman | — | OHKAAL | 1:01.01 | — | — |
| 5 | Chad Nier | 17 | OHSGR | 1:01.47 | B | 422 |
| 6 | Ian Conkel | 15 | OHSNW | 1:01.72 | BB | 467 |
| 1 | Anya Kenny | 14 | OHDR | 1:02.75 | A | 584 |
| 2 | Preslee Cook | 15 | OHCHFY | 1:03.06 | A | 556 |
| 2 | Shealyn Davis | 15 | OHDR | 1:03.67 | A | 540 |
| 6 | Maxwell Medearis | 15 | OHDR | 1:07.22 | B | 361 |
| 3 | Aubrey Hoying | 17 | OHMCY | 1:07.92 | BB | 426 |
| 3 | Madeleine Wolf | 18 | OHCFY | 1:07.99 | BB | 425 |
| 7 | Jackson Heyne | 16 | OHKAAL | 1:08.21 | — | 321 |
| 4 | Renee Dieter | — | OHBVC | 1:10.87 | — | — |
| 5 | Doris Kang | 17 | OHBVC | 1:10.91 | B | 375 |
| 7 | Ronan Moeller | — | OHHHW | 1:17.92 | — | — |
| 6 | Emma Haugan | — | OHXEN | 1:18.10 | — | — |
| 8 | Jethro Hoke | — | OHJGR | 1:19.33 | — | — |
| 9 | Kiefer Livingston | — | OHDCH | 1:21.28 | — | — |
| 4 | Sarah Carter | 16 | OHKEY | 1:28.88 | — | 194 |
| 7 | Lacey Laugle | — | OHHHW | 1:35.86 | — | — |
| 8 | Shelby Smith | — | OHFAI | 1:42.47 | — | — |