| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Paul DeMartini | — | D2FARM | 50.29 | — | — |
| 2 | Lucas Hosch | 17 | D1ROCH | 50.52 | — | 761 |
| 3 | Elliot Rijnovean | — | D2BHS | 50.73 | — | — |
| 4 | Emmett Knudsen | — | D2BHS | 53.82 | — | — |
| 5 | Henry Fokken | 16 | MIOLY | 53.85 | — | 652 |
| 6 | Gonzalo Pena | 18 | MILL | 54.17 | — | 614 |
| 7 | Drew Bronczyk | 16 | D1LKOR | 54.35 | — | 634 |
| 8 | Ryan Dunlap | — | D2BHS | 54.42 | — | — |
| 9 | Gavin D'Alessandro | — | D2BHS | 55.60 | — | — |
| 10 | Keegan Kosiba | — | D2BHS | 56.06 | — | — |
| 11 | Colin Steffens | 17 | D1LKOR | 56.19 | — | — |
| 12 | Jayson Kim | 14 | MIUSSC | 58.13 | BB | 603 |
| 13 | Carter Richmond | — | D2BHS | 58.68 | — | — |
| 14 | Shivaen Myneni | 15 | D1ROCH | 58.92 | BB | 536 |
| — | Brock Kusiak | 16 | D2BHGR | 59.09 | BB | — |
| — | Daniel Mordukhovich | 17 | D2BHGR | 59.38 | — | 469 |
| 15 | Napo Lino | — | D1ROCH | 59.51 | — | — |
| 16 | Bodhi Chinnaiyan | — | D2BHS | 1:00.00 | — | — |
| — | Owen Kleber | 16 | D1RADM | 1:01.71 | — | — |
| — | Kristian Janevski | — | D1LKOR | 1:02.00 | — | — |
| — | Brendan Czapski | — | D1LKOR | 1:03.56 | — | — |
| — | Ibraheem Abukamil | — | D1ROCH | 1:06.14 | — | — |
| — | Patricio Hernandez | — | D2BHS | 1:07.16 | — | — |
| — | Lucas Wells | — | D1LKOR | 1:07.85 | — | — |
| — | Zayaan Ansari | — | D1ROCH | 1:08.34 | — | — |
| — | Carlo Escutia Perez | — | D1ROCH | 1:09.51 | — | — |
| — | Riley Partridge | — | D2FARM | 1:13.67 | — | — |