| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Hannah Farah | 12 | MIUSSC | 40.98 | — | — |
| 2 | Julia Grodzicka | 11 | MIUSSC | 41.67 | — | — |
| 1 | Eduardo Zapata-Yanez | 11 | MIRS | 42.08 | — | — |
| 3 | Melanie He | 12 | MILIFE | 42.62 | — | — |
| 2 | Andrew House | 10 | MIOLY | 42.81 | — | — |
| 3 | William Charlton | 11 | MILIFE | 43.24 | — | — |
| 4 | Marite Pena | 11 | MIRS | 43.31 | — | — |
| 5 | Eva Raina | 11 | MILL | 43.64 | — | — |
| 6 | Kaitlyn Kim | 12 | MILIFE | 45.11 | — | — |
| 7 | Kylie Lohr | 11 | MILL | 46.30 | — | — |
| 8 | Skylar Perry | 9 | MILIFE | 46.81 | — | — |
| 4 | Monty Jackson | 10 | MIRS | 48.08 | — | — |
| 9 | Fiona Bryen | 11 | MIRS | 49.34 | — | — |
| 10 | Kayla Clyne | 9 | MIOLY | 49.47 | — | — |
| 11 | Aleksandra Brodowska | 10 | MILIFE | 49.67 | — | — |
| 12 | Taleen Garamoon | 11 | MIPCC | 49.71 | — | — |
| 5 | Evan Xiao | 10 | MILIFE | 49.92 | — | — |
| 6 | Jayden Kim | 10 | MILIFE | 50.01 | — | — |
| 13 | Kate Yakel | 9 | MIUSSC | 50.16 | — | — |
| 14 | Madeline Mayotte | 9 | MDCAC | 51.73 | — | — |
| 15 | Olivia Zajac | 9 | MIUSSC | 54.01 | — | — |
| 7 | Neil Sandeep | 11 | MILIFE | 55.34 | — | — |
| 16 | Sofia Hernandez-Flores | 10 | MILIFE | 55.56 | — | — |
| 16 | Kinley DeHondt | 10 | MIUSSC | 55.56 | — | — |
| 8 | Brennen McDonald | 9 | MIRS | 55.86 | — | — |
| 18 | Ava Burbank | 12 | MIDASA | 58.39 | — | — |
| 19 | Hibaq Farah | 8 | MIUSSC | 1:00.00 | — | — |
| 20 | Daniella Alsabbagh | 11 | MILIFE | 1:01.71 | — | — |
| 21 | Isabel Bryen | 9 | MIRS | 1:03.14 | — | — |
| 9 | Christian Lohr | 9 | MILL | 1:12.65 | — | — |