| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Boston Buehler | 8 | NCYOTA | 48.89 | — | — |
| 2 | Michael Williams | 7 | NCMOR | 51.11 | — | — |
| 3 | Eric Perry | 8 | NCYOTA | 51.49 | — | — |
| 4 | Colton Blazer | 8 | NCNCAC | 51.63 | — | — |
| 5 | Rachel Kruse | 8 | NCYOTA | 52.55 | — | — |
| 6 | Langston Berryman | 8 | NCYOTA | 52.85 | — | — |
| 7 | Paul Huang | 8 | NCYOTA | 53.08 | — | — |
| 8 | Natasha Susott | 8 | NCTAC | 53.09 | — | — |
| 9 | Christopher Lowther | 7 | NCYOTA | 53.49 | — | — |
| 10 | Macie Perry | 8 | NCYOTA | 53.68 | — | — |
| 11 | Elise Luedke | 8 | NCYOTA | 55.42 | — | — |
| 12 | Craft Baum | 8 | NCMOR | 55.55 | — | — |
| 13 | Katie Cao | 7 | NCYOTA | 57.06 | — | — |
| 14 | Amelia Daenen | 8 | NCYOTA | 58.79 | — | — |
| 15 | Goldie Hyman | 6 | NCYOTA | 59.19 | — | — |
| 16 | James Noeth | 8 | NCYOTA | 1:00.35 | — | — |
| 17 | Tristan Keough | 7 | NCYOTA | 1:00.66 | — | — |
| 18 | June Joiner | 7 | NCYOTA | 1:02.02 | — | — |
| 19 | Nora Taylor | 7 | NCYOTA | 1:02.53 | — | — |
| 20 | Janelle Abebe | 8 | NCYOTA | 1:03.12 | — | — |
| 21 | Cora Messier | 7 | NCYOTA | 1:03.85 | — | — |
| 23 | Annabelle Swenson | 8 | NCYOTA | 1:06.01 | — | — |
| 24 | Alexander Broughton | 8 | NCNCAC | 1:06.62 | — | — |
| 25 | Bianca Zito | 8 | NCYOTA | 1:13.83 | — | — |
| 26 | Roland Pelc | 7 | NCYOTA | 1:14.57 | — | — |
| 27 | Noah Scott | 8 | NCYOTA | 1:17.06 | — | — |
| 28 | John Huang | 6 | NCYOTA | 1:36.48 | — | — |