| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Caroline Bryan | 14 | MIGPG | 55.47 | AAAA | 845 |
| 2 | Hannah DiDio | 17 | D2GPS | 59.47 | — | 635 |
| 3 | Madison Johannes | 17 | MIGLT | 1:02.53 | A | 546 |
| 4 | Lillian Irby | 17 | D2GPS | 1:02.93 | A | — |
| 5 | Cailey Hard | — | D2GPN | 1:03.78 | — | — |
| 9 | Maya Bowman | 17 | D1UIKE | 1:04.45 | BB | 499 |
| 6 | Erin O'Rourke | 14 | MIUSSC | 1:04.48 | A | 538 |
| 7 | Maria Melhem | — | D2GPN | 1:04.58 | — | — |
| 8 | Lorelei Carr | 16 | MIGPG | 1:04.94 | BB | 497 |
| — | Kate Dixon | 18 | D2GPS | 1:04.99 | — | 487 |
| 10 | Elaina Mowid | 16 | MIMOST | 1:08.49 | BB | 424 |
| — | Samantha Turner | 16 | D2GPS | 1:08.64 | B | — |
| 11 | Alyssa Jutzy | — | D1MDAK | 1:10.10 | — | — |
| 12 | Ayla Grazioli | — | D2GPN | 1:14.21 | — | — |
| 13 | Aubrey Killian | 16 | D1UIKE | 1:15.57 | — | 316 |
| 14 | Sharon Martin | 16 | D1MLCN | 1:16.23 | — | 307 |
| 15 | Amy Neumann | — | D1MDAK | 1:22.17 | — | — |
| — | Cassidy Cleland | 15 | D2GPS | 1:23.84 | — | 236 |
| 16 | Riley Muirhead | — | D1MDAK | 1:25.82 | — | — |
| — | Abigail Kelly | 15 | D1UIKE | 1:51.31 | — | — |