| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Antonio Belleza | — | D1PLYM | 52.71 | — | — |
| 2 | Justin Okopny | 14 | MICW | 54.42 | AA | 769 |
| 3 | Colin Domeracki | 17 | D1NORV | 54.51 | — | 641 |
| 4 | Anthony Chen | — | D1PLYM | 54.64 | — | — |
| 5 | Gries Kane | 18 | D1BRI | 55.54 | — | 593 |
| 9 | Micah Styles | 16 | D1CANT | 55.94 | A | 618 |
| 6 | Ryan Emanuel | — | D1NOVI | 56.13 | — | — |
| 10 | Cian Ziembroski | 16 | MILCSC | 56.44 | A | 602 |
| 7 | Daniel Shi | 17 | D1SALE | 56.46 | — | 577 |
| 8 | Clive Smith | 16 | MISLA | 56.90 | A | 587 |
| 11 | Arnav Kulkarni | 16 | MILCSC | 58.10 | BB | 552 |
| 12 | Shenhao Deng | 17 | D1CANT | 59.66 | BB | 489 |
| 13 | Venu Ganti | — | D1PLYM | 59.84 | — | — |
| 14 | Rohan Patil | — | D1NOVI | 1:00.03 | — | — |
| 15 | Jude Adkins | 16 | D1SALE | 1:00.23 | BB | 495 |
| 17 | Alexander Feng | 16 | D1NORV | 1:00.73 | BB | 483 |
| 18 | Benjamin Sevener | 15 | MIPCC | 1:01.16 | BB | 506 |
| 19 | Leonidas Fainekos-ishiwata | — | D1NOVI | 1:01.17 | — | — |
| 16 | Kaden Delaney | 17 | MIPCC | 1:01.62 | B | 444 |
| 20 | Hariswaran Balamurugan | — | D1PLYM | 1:02.52 | — | — |
| 21 | Grant Henson | 18 | D1HTLD | 1:03.00 | — | — |
| 22 | Josh Sevener | 16 | D1SALE | 1:03.40 | B | 425 |
| 23 | Francesco Marra | 16 | MIPCC | 1:03.89 | B | 415 |
| — | Conan Shinohara | — | D1NOVI | 1:04.29 | — | — |
| — | Harneil Banga | — | D1PLYM | 1:04.30 | — | — |
| 24 | Luke O'Brian | — | D1BRI | 1:04.93 | — | — |