| Place | Swimmer | Age | Club | Time | Std | Power pts |
|---|---|---|---|---|---|---|
| 1 | Addison Forbes | 17 | MINKAT | 56.22 | — | 825 |
| 2 | Brooklyn Beauch | 17 | MIUN | 56.49 | — | 813 |
| 3 | Rutaja Nadgauda | — | D1FHNE | 1:00.91 | — | — |
| 4 | Meredith Sperling | — | D3EGR | 1:02.70 | — | — |
| 5 | Nora Magers | 17 | MIEGRA | 1:02.77 | — | 593 |
| 9 | Kaitlyn Goins | — | D3EGR | 1:03.10 | — | — |
| 6 | Margaret Holt | 15 | D2GRFH | 1:03.16 | A | 590 |
| 10 | Taylor Blomeling | 13 | D2BYC | 1:03.51 | A | — |
| 11 | Lucy Haney | 15 | D2BYC | 1:03.79 | A | 572 |
| 7 | Avery Lowe | — | D2GRN | 1:04.11 | — | — |
| 12 | Juliana Mills | — | MIGRCH | 1:05.66 | — | — |
| 8 | Ellie Palmer | 14 | D2GRFH | 1:05.84 | BB | — |
| 13 | Sam Williams | — | D3EGR | 1:06.29 | — | — |
| 14 | Olivia Miller | — | D3EGR | 1:06.45 | — | — |
| 15 | Cyan Hayes | — | D1FHNE | 1:07.68 | — | — |
| 17 | Avery Lowe | — | D3EGR | 1:09.36 | — | — |
| 16 | Isabella Nibbelink | — | D2GRN | 1:09.58 | — | — |
| 18 | Simiana Tyrrell | — | D2GRFH | 1:12.21 | — | — |
| 19 | Margaret McIntosh | — | D3EGR | 1:12.72 | — | — |
| 20 | Micah Heerema | — | MIGRCH | 1:15.32 | — | — |
| 21 | Vivian Ralya | — | D1FHNE | 1:16.03 | — | — |
| 22 | Evie Wing | — | D3EGR | 1:18.27 | — | — |
| 23 | Karson VanDenened | — | MIGRCH | 1:20.16 | — | — |
| 24 | Miriam Kuperus | — | MIGRCH | 1:20.29 | — | — |
| — | Kate Boyer | — | D2BYC | 1:40.58 | — | — |